“Most chemical feedstocks come from petroleum and natural gas, and we need other sources,” said Shota Atsumi, assistant professor of chemistry at UC Davis and lead author of the study published Jan. 7 in the Proceedings of the National Academy of Sciences. The U.S. Department of Energy has set a goal of obtaining a quarter of industrial chemicals from biological processes by 2025. Biological reactions are good at forming carbon-carbon bonds, using carbon dioxide as a raw material for reactions powered by sunlight. It’s called photosynthesis, and cyanobacteria, also known as “blue-green algae,” have been doing it for more than 3 billion years. Using cyanobacteria to grow chemicals has other advantages: They do not compete with food needs, like corn’s role in the creation of ethanol. The challenge is to get the cyanobacteria to make significant amounts of chemicals that can be readily converted to chemical feedstocks. With support from Japanese chemical manufacturer Asahi Kasei Corp., Atsumi’s lab at UC Davis has been working on introducing new chemical pathways into the cyanobacteria. The researchers identified enzymes from online databases that carried out the reactions they were looking for, and then introduced the DNA for these enzymes into the cells. Working a step at a time, they built up a three-step pathway that allows the cyanobacteria to convert carbon dioxide into 2,3 butanediol, a chemical that can be used to make paint, solvents, plastics and fuels. Because enzymes may work differently in different organisms, it is nearly impossible to predict how well the pathway will work before testing it in an experiment, Atsumi said. After three weeks growth, the cyanobacteria yielded 2.4 grams of 2,3 butanediol per liter of growth medium — the highest productivity yet achieved for chemicals grown by cyanobacteria and with potential for commercial development, Atsumi said. Atsumi hopes to tune the system to increase productivity further and experiment with other products, while corporate partners explore scaling up the technology. Co-authors of the paper are graduate student John Oliver, postdoctoral researcher Iara Machado and Hisanari Yoneda, a visiting researcher from Asahi Kasei Corp.
About UC Davis
For more than 100 years, UC Davis has engaged in teaching, research and public service that matter to California and transform the world. Located close to the state capital, UC Davis has more than 33,000 students, more than 2,500 faculty and more than 21,000 staff, an annual research budget of nearly $750 million, a comprehensive health system and 13 specialized research centers. The university offers interdisciplinary graduate study and more than 100 undergraduate majors in four colleges — Agricultural and Environmental Sciences, Biological Sciences, Engineering, and Letters and Science. It also houses six professional schools — Education, Law, Management, Medicine, Veterinary Medicine and the Betty Irene Moore School of Nursing. Source: University of California, Davis
